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147![RNAi by feeding 2 Steps 1. Transform L4440-XX plasmid into HT115 (DE3) bacteria – plate on LB/amp/tet 2. Inoculate 50 ml LB/amp/tet w. 0.5 ml O/N culture or directly from plate 3. Grow at 37°C until OD600 reaches 0.4 RNAi by feeding 2 Steps 1. Transform L4440-XX plasmid into HT115 (DE3) bacteria – plate on LB/amp/tet 2. Inoculate 50 ml LB/amp/tet w. 0.5 ml O/N culture or directly from plate 3. Grow at 37°C until OD600 reaches 0.4](https://www.pdfsearch.io/img/93acddbd68e0297820a9f5e1ebdaed32.jpg) | Add to Reading ListSource URL: hymanlab.mpi-cbg.de- Date: 2012-09-17 09:29:00
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